| 01/02/08 | NAC Zwaanswijk 21(p) | 1-0 | Twente |
| 02/02/08 | Heerenveen Sulejmani 10, Pranjic 14, Bradley 50, Zuiverloon 58, Beerens 66, 80, Prager 82 | 7-0 | Vitesse |
| 02/02/08 | AZ | 0-2 | PSV Lazovic 13, Afellay 25 |
| 02/02/08 | Excelsior Luyckx 84 | 1-1 | De Graafschap Tarvajarvi 23 |
| 02/02/08 | Heracles | 0-2 | NEC Lens 26, 68 |
| 03/02/08 | Ajax Heitinga 8, Huntelaar 45, 90 | 3-0 | Feyenoord |
| 03/02/08 | Groningen Powel 15 | 1-1 | Roda Oper 81 |
| 03/02/08 | Utrecht Van der Gun 4, Nelisse 56 | 2-0 | Willem II |
| 03/02/08 | Sparta Promes 45, Rose 49 | 2-0 | VVV |
| 06/02/08 | Sparta | 0-1 | NAC Ammi 63 |
| 08/02/08 | Twente Elia 77, N'Kufo 86 | 2-1 | Heracles Gonzalo 73 |
| 09/02/08 | PSV Bakkal 1 | 1-1 | Heerenveen Bradley 52 |
| 09/02/08 | NAC Elshot 2 | 1-0 | Utrecht |
| 09/02/08 | De Graafschap Schone 18 | 1-3 | Feyenoord Makaay 45, Hofs 56, 63 |
| 09/02/08 | Vitesse Gommans 34, Van der Schaaf 75 | 2-1 | Groningen Meerdink 56 |
| 10/02/08 | Roda Lamah 22, Hadouir 79 | 2-1 | Ajax Huntelaar 38 |
| 10/02/08 | VVV Reekers 45 | 1-1 | Willem II Cristiano 77 |
| 10/02/08 | Sparta | 0-1 | Excelsior van Steensel 58 |
| 10/02/08 | NEC Bobson 10, Holman 13, van Beukering 20(p), Wisgerhof 24, 24, Drost 68 | 5-2 | AZ Mendes Da Silva 3, El Hamdaoui 88 |
| 15/02/08 | Ajax Heitinga 14, 39, Huntelaar 29, Lindgren 31, Perez 40, 81 | 6-2 | Sparta Roberts 34, Emnes 82 |
| 16/02/08 | Roda Lamah 24, Janssen 32, Van Tornhout 62 | 3-1 | Twente N'Kufo 33 |
| 16/02/08 | Willem II | 0-1 | PSV Simons 83(p) |
| 16/02/08 | Heracles Gonzalo 20, Schilder 80 | 2-2 | Heerenveen Beerens 18, Sulejmani 30 |
| 16/02/08 | NEC | 0-1 | Excelsior Den Ouden 71 |
| 17/02/08 | Utrecht Wolters 89 | 1-4 | VVV Calabro 17(p), 80, Oost 39, 49 |
| 17/02/08 | Feyenoord De Guzman 45 | 1-0 | Vitesse |
| 17/02/08 | Groningen Berg 30, 86(p) | 2-1 | AZ Jaliens 83 |
| 17/02/08 | De Graafschap | 0-1 | NAC Molhoek 76 |
| 22/02/08 | Sparta Emnes 39, Boukhari 57 | 2-1 | Utrecht Kruys 56 |
| 23/02/08 | AZ | 0-0 | Twente |
| 23/02/08 | Excelsior | 0-0 | Willem II |
| 23/02/08 | Heracles Ramos da Silva 1, Lakic 27, 60(p) | 3-3 | Feyenoord Andre Bahia 17, Landzaat 31, Hofs 57 |
| 23/02/08 | Vitesse Janssen 67 | 1-1 | Roda Saeijs 87 |
| 24/02/08 | NAC Amoah 50, Penders 58 | 2-3 | Ajax Huntelaar 20, Emanuelson 76, Heitinga 82 |
| 24/02/08 | VVV Leemans 50, Oost 60 | 2-5 | Groningen Lovre 16, Berg 45, 85, Powel 47, Niljand 89 |
| 24/02/08 | PSV Lazovic 8, 90, Bakkal 52, Simons 82(p) | 4-1 | De Graafschap Schone 7 |
| 24/02/08 | Heerenveen Beerens 2, Arend Roorda 29 | 2-3 | NEC Lens 63, Holman 73, El Akchaoui 90 |
| 29/02/08 | Vitesse Kolk 60 | 1-3 | VVV Oost 2(p), Amrabat 6, 45 |
